Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common Pitfalls from Cell Seeding to Analysis

The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of experimental biology. Reproducibility of cell-based assays is one of the key points when it comes to data reliability. Despite choosing the appropriate assay type there are many more factors to be considered to mitigate data variability. This joint Promega and Eppendorf webinar series gives a comprehensive overview of the cell culture workflow and introduce measures to maximize the outcome of your cell-based assay.

In this episode, our experts will review the most frequently asked questions, that have been collected over the past years. 

Join us to participate in the live Q&A session. If you can′t make it just register to receive the link to the recorded session.

Key Topics:

  • When to ideally time an analysis?
  • Does the passage number influence experimental outcomes?
  • When to choose what detection mode?
  • How to select the appropriate microtiter plate?
  • What is the best method for detecting mycoplasma?

Date & Time:
November 18, 2020 at 4:00pm CET, 10:00am EST, 07:00am PST

Jessica Wagener, Ph.D., Application Specialist Cell Handling, Eppendorf AG
Erik Bonke, Ph.D., Application Specialist, Promega GmbH

Register here

Jessica Wagener, Ph.D
Erik Bonke, Ph.D

Optimize your bioprocess – From shaker to bioreactor

Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of production often involves shake flasks and bioreactors. Shake flasks are used for bioreactor inoculum production and are an easy-to-use instrument for early process development like organism screening and media design. At the next process steps, highly controllable bioreactors are needed for flexible solutions, automated process control, and predictable scalability when larger quantities of cells are required. The challenge during upscaling is to keep a constant efficiency and quality during all process steps.

In this webinar, we will depict the typical applications of shakers and bioreactors in an upscaling bioprocess. We will highlight applicational differences and factors that influence cultivation and system transition. Furthermore, we will introduce to you the basic components of a bioreactor and the various process parameters that can be controlled. Finally, we will go through different process strategies to further optimize a process and increase production.

Target groups:

  •  Beginners, who start using shake flasks and bioreactors for cultivation and upscaling
  •  Early bioreactor users, who want to deepen their knowledge and understanding of the possibilities of process control strategies

Join live for a Q&A session with experts!

Scheduling conflict?

If you can′t make it, just register to receive the link to the recorded session.

In this webinar you will:

  •  Learn about typical applications of shake flasks and stirred-tank bioreactors in bioprocess development
  •  Learn about influencing factors and optimizing setscrews in shaking/stirring
  •  Learn about the basic bioreactor components and the different process parameters that can be monitored and controlled, and what they tell you about your culture
  •  Learn about feeding possibilities and their impact on your culture

Date & Time
December 2, 2020 at 08:00 am PDT, 11:00 am EDT, 5:00 pm CEWT

Ines Hartmann, Application Specialist Cell Handling, Eppendorf AGDavid Solbach, Scientific Communications Manager, Eppendorf Bioprocess Center

Register here

Ines Hartmann, Application Specialist Cell Handling, Eppendorf AG
David Solbach, Scientific Communications Manager, Eppendorf Bioprocess Center